Evaluation of antioxidant status and oxidative stress in sheep experimentally infected with Anaplasma ovis

Authors

  • A. Gharabaghi Resident of Large Animal Internal Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • B. Kazemi Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • H. Shaygan Graduated from College of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
  • J. Salar Amoli Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • S. M. Jalali Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • S. P. Yasini Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • T. Ali Esfehani BSc in Laboratory Sciences, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Z. Khaki Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:

Anaplasma ovis infections can cause severe anemia in the acute phase of the disease. In order to investigate the alterations of erythrocyte protective antioxidant mechanisms associated with anemia in sheep experimentally infected with A. ovis, 100 ml heparinized blood was collected from splenectomised sheep that showed 6% A. ovis parasitemia. Inoculums of 20 ml blood were administered intravenously to five male sheep without any blood parasite. Parasitological and haematological changes and the activities of erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were studied inexperimentally infected animals on the 0-38 post infection days. Parasitemia increased significantly with the progress of infection and reached its maximum level on day 15 of the experiment. From this point to day 38, there was a gradual decline in parasitemia. A significant decrease in PCV, RBC and Hb concentration was evident coincidentally with peak parasitaemia in the infected sheep. On post infection day 15, the activities of all enzymes increased, the changes being significant for SOD activity. There was a significant positive correlation among parasitemia and the activities of erythrocyte SOD (r = 0.644, P

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Journal title

volume 15  issue 1

pages  50- 53

publication date 2014-03-30

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